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KMID : 0861020170320020025
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Korea Journal of Herbology
2017 Volume.32 No. 2 p.25 ~ p.32
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Anti-inflammatory Effects of Ethanol Extract of Korean Medicinal Plants at Hwaak Mountain in LPS-induced RAW 264.7 Macrophages
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Kang Yun-Mi
Jeon Eun-Jin
Chung Kyung-Sook
Cheon Se-Yun
Park Jong-Hyuk
Han Yoo-Chang
An Hyo-Jin
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Abstract
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Objectives : This study was conducted to investigate candidate materials as anti-inflammatory agent from extracts of Korean medicinal plants in Hwaak mountain. Ligustrum obtusifolium (LO) is a Korea medicinal plants that commonly used for robustness and hemostasis. It has been reported that LO has exhibited anti-ischemic, anti-oxidative, antihypolipidemic, anti-tumor and hypoglycemic effects. However, LO has not been previously reported to have an antiinflammatory effect. Therefore, we have evaluated the anti-inflammatory effects of LO and its underlying molecular mechanisms in LPS-induced RAW 264.7 macrophages.
Methods : Cell viability was determined by MTT assay in RAW 264.7 macrophages. Nitric Oxide (NO) was measured with Griess reagent and pro-inflammatory cytokines were detected by ELISA in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Protein expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and p65 subunit of nuclear factor-κB (NF-κB) were determined by Western blot analysis.
Results : Among 15 extracts of Korean medicinal plants tested, Ligustrum obtusifolium (LO) showed the inhibition of NO production without cytotoxicity. LO reduced the expression levels of iNOS and COX-2 proteins in LPS-simulated RAW 264.7 macrophages in dose-dependent manner. Consistent with these data, LO inhibited the productions of TNF-α, IL-6, and IL-1β in LPS-simulated RAW 264.7 macrophages. Furthermore, LO attenuated the LPS-induced nuclear translocation of p65 NF-κB in RAW 264.7 macrophages involving suppression of NF-κB activation.
Conclusions : Taken together, these results suggest that the anti-inflammatory effects of LO is associated with regulation of inflammatory mediators via inhibition of NF-κB activation in LPS-treated RAW 264.7 macrophages.
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KEYWORD
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Ligustrum obtusifolium (LO), lipopolysaccharide (LPS), nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-κB (NF-κB)
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